Exploring the Early Diagnostic Value of Balf tNGS Detection in Bronchoalveolar Lavage Fluid for NTM-PD Combined with Pulmonary Mycosis

WU Ruiyan; CHEN Pinru; TAN Yaoju

doi:10.15912/j.issn.1671-8194.2025.12.007

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Guide of China Medicine ›› 2025, Vol. 23 ›› Issue (12) : 29-31. DOI: 10.15912/j.issn.1671-8194.2025.12.007

Exploring the Early Diagnostic Value of Balf tNGS Detection in Bronchoalveolar Lavage Fluid for NTM-PD Combined with Pulmonary Mycosis

WU Ruiyan^*, CHEN Pinru, TAN Yaoju

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Abstract

Objective To investigate the value of pathogen targeted sequencing in alveolar lavage fluid (Balf tNGS) in early diagnosis of non-tuberculous mycobacterium lung disease (NTM-PD) complicated with pulmonary mycosis. Methods BALF samples were collected from 67 suspected NTM-PD patients with pulmonary mycosis who showed imaging deterioration at our hospital for tNGS detection and fungal culture identification, and the results were compared and evaluated. Results A total of 56 fungi were detected by Balf tNGS, the detection rate was 83.58%, and the top 3 fungi were Aspergillus fumigatus, Candida albicans and Sedospora. The results of paired Chi-square test (P<0.001) were significant, indicating that tNGS detection was significantly superior to fungal culture identification. Conclusions BALF tNGS can provide a pathogenic diagnostic reference for cases of NTM-PD imaging deterioration, and is of positive significance for early diagnosis of pulmonary mycosis.

Key words

Non tuberculosis mycobacterial disease / Pulmonary mycosis / Bronchoalveolar lavage fluid

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WU Ruiyan, CHEN Pinru, TAN Yaoju. Exploring the Early Diagnostic Value of Balf tNGS Detection in Bronchoalveolar Lavage Fluid for NTM-PD Combined with Pulmonary Mycosis[J]. Guide of China Medicine. 2025, 23(12): 29-31 https://doi.org/10.15912/j.issn.1671-8194.2025.12.007

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