Objective To analyze HBV-DNA load and its relationship with liver fibrosis and liver function in chronic hepatitis B virus (HBV) infected patients. Methods Seventy-eight patients hospitalized with chronic hepatitis B admitted to the inpatient department of Zhongshan Hospital, Fudan University (Xiamen Branch), from August 2017 to October 2022 were selected for the study. The fluorescence quantitative polymerase chain reaction method was used to detect serum HBV-DNA load, and the patients of HBV-DNA load≥100 IU / mL were assigned in the study group, and the patients of HBV-DNA load<100 IU / mL for the control group. Then liver fibrosis indexes and the functional performance indexes, and Spearman correlation analysis was used to analyze the relationship between HBV-DNA loads and hepatic fibrosis and functional performance indicators. Results Patients in the study group had higher values of ALT, AST, TBIL, DBIL, γ-GT, ALP, and TBA than those in the control group (P<0.05), and higher levels of PC III, IV-C, LN, and HA than those in the control group (P<0.05). The results of the Spearman's correlation analysis revealed that patients with chronic hepatitis B HBV-DNA load were positively correlated with liver function indexes ALT, AST, TBIL,and liver fibrosis indexes DBIL, PCⅢ, IV-C, LN (P<0.05). Conclusions There is a close relationship between HBV-DNA viral load, liver fibrosis ,and liver function in patients with chronic hepatitis B. It is expected to improve the diagnostic efficacy of the disease by combining the detection of HBV-DNA viral load and liver fibrosis, and the clinic can evaluate the progress of liver fibrosis and the severity of the disease accordingly.